Use of the human colorectal adenocarcinoma (Caco‐2) cell line for isolating respiratory viruses from nasopharyngeal aspirates
Identifieur interne : 001A37 ( Main/Exploration ); précédent : 001A36; suivant : 001A38Use of the human colorectal adenocarcinoma (Caco‐2) cell line for isolating respiratory viruses from nasopharyngeal aspirates
Auteurs : K. H. Chan [République populaire de Chine, Hong Kong] ; M. K. Yan [République populaire de Chine] ; K. K. W. To [République populaire de Chine] ; S. K. Lau [République populaire de Chine] ; P. C. Woo [République populaire de Chine] ; V. C. C. Cheng [République populaire de Chine] ; W. S. Li [République populaire de Chine] ; J. F. W. Chan [République populaire de Chine] ; H. Tse [République populaire de Chine] ; K. Y. Yuen [République populaire de Chine]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2013-05.
English descriptors
- Teeft :
- Administrative region, Antigen testing, Aspirate, Caco2 cells, Cell culture, Cell cultures, Cell line, Cell lines, Cell pellet, Chan, Clin, Clin virol, Clinical samples, Conventional cell line panel, Conventional cell lines, Conventional cell panel, Conventional panel, Culture tubes, Different cell lines, Different combinations, Direct antigen, Direct antigen test, Enteric viruses, Hong kong, Human virus, Mdck, Mdck cells, Monoclonal antibodies, Nasopharyngeal, Nasopharyngeal aspirate, Nasopharyngeal aspirate sample, Nasopharyngeal aspirate samples, Nasopharyngeal aspirate specimens, Nasopharyngeal aspirates, Panel cells, Positive cultures, Queen mary hospital, Rapid diagnosis, Respiratory diseases, Respiratory syncytial virus, Respiratory viruses, Sars coronavirus, Tract infections, Typing reagents, Viral, Virol, Virol methods, Virus, Virus type, Wiley periodicals.
Abstract
The human colorectal adenocarcinoma‐derived Caco‐2 cell line was evaluated as a means isolating common respiratory viruses from nasopharyngeal aspirates for the diagnosis of respiratory diseases. One hundred eighty‐nine direct immunofluorescence positive nasopharyngeal aspirates obtained from patients with various viral respiratory diseases were cultured in the presence of Caco‐2 cells or the following conventional cell lines: LLC‐MK2, MDCK, HEp‐2, and A549. Caco‐2 cell cultures effectively propagated the majority (84%) of the viruses present in nasopharyngeal aspirate samples compared with any positive cultures obtained using the panel cells (78%) or individual cell line MDCK (38%), HEp‐2 (21%), LLC‐MK2 (27%), or A549 (37%) cell lines. The differences against individual cell line were statistically significant (P = < 0.000001). Culture in Caco‐2 cells resulted in the isolation of 85% (36/42) of viruses which were not cultivated in conventional cell lines. By contrast, 80% (24/30) of viruses not cultivated in Caco‐2 cells were isolated using the conventional panel. The findings indicated that Caco‐2 cells were sensitive to a wide range of viruses and can be used to culture a broad range of respiratory viruses. J. Med. Virol. 85:874–879, 2013. © 2013 Wiley Periodicals, Inc.
Url:
DOI: 10.1002/jmv.23538
Affiliations:
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Cheng</name></author><author><name sortKey="Li, W S" sort="Li, W S" uniqKey="Li W" first="W. S." last="Li">W. S. Li</name></author><author><name sortKey="Chan, J F W" sort="Chan, J F W" uniqKey="Chan J" first="J. F. W." last="Chan">J. F. W. Chan</name></author><author><name sortKey="Tse, H" sort="Tse, H" uniqKey="Tse H" first="H." last="Tse">H. Tse</name></author><author><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K. Y." last="Yuen">K. Y. 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H." last="Chan">K. H. Chan</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Hong Kong</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Hong Kong</country><wicri:regionArea>Correspondence address: Department of Microbiology, University Pathology Building, Queen Mary Hospital, Pokfulam</wicri:regionArea><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation></author><author><name sortKey="Yan, M K" sort="Yan, M K" uniqKey="Yan M" first="M. K." last="Yan">M. K. Yan</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="To, K K W" sort="To, K K W" uniqKey="To K" first="K. K. W." last="To">K. K. W. To</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Lau, S K" sort="Lau, S K" uniqKey="Lau S" first="S. 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Woo</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, V C C" sort="Cheng, V C C" uniqKey="Cheng V" first="V. C. C." last="Cheng">V. C. C. Cheng</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Li, W S" sort="Li, W S" uniqKey="Li W" first="W. S." last="Li">W. S. Li</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Chan, J F W" sort="Chan, J F W" uniqKey="Chan J" first="J. F. W." last="Chan">J. F. W. Chan</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Tse, H" sort="Tse, H" uniqKey="Tse H" first="H." last="Tse">H. Tse</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K. Y." last="Yuen">K. Y. Yuen</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">85</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="874">874</biblScope><biblScope unit="page" to="879">879</biblScope><biblScope unit="page-count">6</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2013-05">2013-05</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Administrative region</term><term>Antigen testing</term><term>Aspirate</term><term>Caco2 cells</term><term>Cell culture</term><term>Cell cultures</term><term>Cell line</term><term>Cell lines</term><term>Cell pellet</term><term>Chan</term><term>Clin</term><term>Clin virol</term><term>Clinical samples</term><term>Conventional cell line panel</term><term>Conventional cell lines</term><term>Conventional cell panel</term><term>Conventional panel</term><term>Culture tubes</term><term>Different cell lines</term><term>Different combinations</term><term>Direct antigen</term><term>Direct antigen test</term><term>Enteric viruses</term><term>Hong kong</term><term>Human virus</term><term>Mdck</term><term>Mdck cells</term><term>Monoclonal antibodies</term><term>Nasopharyngeal</term><term>Nasopharyngeal aspirate</term><term>Nasopharyngeal aspirate sample</term><term>Nasopharyngeal aspirate samples</term><term>Nasopharyngeal aspirate specimens</term><term>Nasopharyngeal aspirates</term><term>Panel cells</term><term>Positive cultures</term><term>Queen mary hospital</term><term>Rapid diagnosis</term><term>Respiratory diseases</term><term>Respiratory syncytial virus</term><term>Respiratory viruses</term><term>Sars coronavirus</term><term>Tract infections</term><term>Typing reagents</term><term>Viral</term><term>Virol</term><term>Virol methods</term><term>Virus</term><term>Virus type</term><term>Wiley periodicals</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The human colorectal adenocarcinoma‐derived Caco‐2 cell line was evaluated as a means isolating common respiratory viruses from nasopharyngeal aspirates for the diagnosis of respiratory diseases. One hundred eighty‐nine direct immunofluorescence positive nasopharyngeal aspirates obtained from patients with various viral respiratory diseases were cultured in the presence of Caco‐2 cells or the following conventional cell lines: LLC‐MK2, MDCK, HEp‐2, and A549. Caco‐2 cell cultures effectively propagated the majority (84%) of the viruses present in nasopharyngeal aspirate samples compared with any positive cultures obtained using the panel cells (78%) or individual cell line MDCK (38%), HEp‐2 (21%), LLC‐MK2 (27%), or A549 (37%) cell lines. The differences against individual cell line were statistically significant (P = < 0.000001). Culture in Caco‐2 cells resulted in the isolation of 85% (36/42) of viruses which were not cultivated in conventional cell lines. By contrast, 80% (24/30) of viruses not cultivated in Caco‐2 cells were isolated using the conventional panel. The findings indicated that Caco‐2 cells were sensitive to a wide range of viruses and can be used to culture a broad range of respiratory viruses. J. Med. Virol. 85:874–879, 2013. © 2013 Wiley Periodicals, Inc.</div></front></TEI><affiliations><list><country><li>Hong Kong</li><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Chan, K H" sort="Chan, K H" uniqKey="Chan K" first="K. H." last="Chan">K. H. Chan</name></noRegion><name sortKey="Chan, J F W" sort="Chan, J F W" uniqKey="Chan J" first="J. F. W." last="Chan">J. F. W. Chan</name><name sortKey="Cheng, V C C" sort="Cheng, V C C" uniqKey="Cheng V" first="V. C. C." last="Cheng">V. C. C. Cheng</name><name sortKey="Lau, S K" sort="Lau, S K" uniqKey="Lau S" first="S. K." last="Lau">S. K. Lau</name><name sortKey="Lau, S K" sort="Lau, S K" uniqKey="Lau S" first="S. K." last="Lau">S. K. Lau</name><name sortKey="Lau, S K" sort="Lau, S K" uniqKey="Lau S" first="S. K." last="Lau">S. K. Lau</name><name sortKey="Lau, S K" sort="Lau, S K" uniqKey="Lau S" first="S. K." last="Lau">S. K. Lau</name><name sortKey="Li, W S" sort="Li, W S" uniqKey="Li W" first="W. S." last="Li">W. S. Li</name><name sortKey="To, K K W" sort="To, K K W" uniqKey="To K" first="K. K. W." last="To">K. K. W. To</name><name sortKey="To, K K W" sort="To, K K W" uniqKey="To K" first="K. K. W." last="To">K. K. W. To</name><name sortKey="To, K K W" sort="To, K K W" uniqKey="To K" first="K. K. W." last="To">K. K. W. To</name><name sortKey="To, K K W" sort="To, K K W" uniqKey="To K" first="K. K. W." last="To">K. K. W. To</name><name sortKey="Tse, H" sort="Tse, H" uniqKey="Tse H" first="H." last="Tse">H. Tse</name><name sortKey="Tse, H" sort="Tse, H" uniqKey="Tse H" first="H." last="Tse">H. Tse</name><name sortKey="Tse, H" sort="Tse, H" uniqKey="Tse H" first="H." last="Tse">H. Tse</name><name sortKey="Tse, H" sort="Tse, H" uniqKey="Tse H" first="H." last="Tse">H. Tse</name><name sortKey="Woo, P C" sort="Woo, P C" uniqKey="Woo P" first="P. C." last="Woo">P. C. Woo</name><name sortKey="Woo, P C" sort="Woo, P C" uniqKey="Woo P" first="P. C." last="Woo">P. C. Woo</name><name sortKey="Woo, P C" sort="Woo, P C" uniqKey="Woo P" first="P. C." last="Woo">P. C. Woo</name><name sortKey="Woo, P C" sort="Woo, P C" uniqKey="Woo P" first="P. C." last="Woo">P. C. Woo</name><name sortKey="Yan, M K" sort="Yan, M K" uniqKey="Yan M" first="M. K." last="Yan">M. K. Yan</name><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K. Y." last="Yuen">K. Y. Yuen</name><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K. Y." last="Yuen">K. Y. Yuen</name><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K. Y." last="Yuen">K. Y. Yuen</name><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K. Y." last="Yuen">K. Y. Yuen</name></country><country name="Hong Kong"><noRegion><name sortKey="Chan, K H" sort="Chan, K H" uniqKey="Chan K" first="K. H." last="Chan">K. H. Chan</name></noRegion><name sortKey="Chan, K H" sort="Chan, K H" uniqKey="Chan K" first="K. H." last="Chan">K. H. Chan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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